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Double-stranded sperm DNA damage is a cause of delay in …
https://www.sciencedirect.com/science/article/pii/S0015028218322362
WEBApr 1, 2019 · To analyze the effect of single- and double-stranded sperm DNA fragmentation (ssSDF and dsSDF) on human embryo kinetics monitored under a time-lapse system.
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Breaks and bends in sperm DNA: their impact on the future
https://www.fertstert.org/article/S0015-0282(19)30008-1/pdf
WEBvision. The results suggested that dsSDF could be the main type of DNA damage affecting embryo kinetics. Available data showed a delay in embryo kinetics and diminished im-plantation rates when a high rate of dsSDF was seen in the semen sample used for ICSI. In contrast, a high rate of ssSDF did not cause any significant effect either on ...
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Double-stranded sperm DNA damage is a cause of delay in …
https://pubmed.ncbi.nlm.nih.gov/30826116/
WEBThe results show that dsSDF could be the main type of DNA damage that affects embryo development in intracytoplasmic sperm injection cycles, probably due to motility-based sperm selection in this assisted reproduction procedure. Conclusion (s): Double-stranded sperm DNA damage caused a delay in embryo development and impaired implantation ...
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Sperm DNA Fragmentation: A New Guideline for Clinicians
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7502318/
WEBAug 6, 2020 · 1. Primary mechanisms underlying sperm DNA fragmentation. SDF is primarily induced by defective maturation and abortive apoptosis occurring within the testis, or by OS throughout the male reproductive tract [].During spermatogenesis, chromatin is compacted through histone exchange with transitional proteins and protamines [].This is …
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Breaks and bends in sperm DNA: their impact on the future of the …
https://www.fertstert.org/article/S0015-0282(19)30008-1/fulltext
WEBSperm DNA fragmentation (SDF) can be single-stranded (ssSDF) or double-stranded (dsSDF). There are numerous assays, such as terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) by flowcytometry or fluorescence microscopy, sperm chromatin structure assay (SCSA), sperm chromatin dispersion (SCD), and Comet assay, available …
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Potential pathways for dsSDF-mediated homologous
https://www.researchgate.net/figure/Potential-pathways-for-dsSDF-mediated-homologous-exchange-SDF-repair-of-chromosomal_fig3_50591611
WEBPotential pathways for dsSDF-mediated homologous exchange: SDF repair of chromosomal DSBs. DSBs [eg, nuclease-induced (yellow) as well as replication anomalies and damage caused by physical or...
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Sperm DNA fragmentation and microfluidics: A new era in human …
https://www.sciencedirect.com/science/article/pii/S2340932022000093
WEBSep 1, 2022 · Sperm DNA fragmentation (SDF) refers to breaks in the genetic material of the male gamete, distinguishing two types: single strand SDF (ssSDF) and double strand SDF (dsSDF) (Agarwal et al., 2020). It has been described that 11% of men present semen values within the normal range established by the World Health Organization (WHO) …
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Double stranded sperm DNA breaks, measured by Comet assay, …
https://pubmed.ncbi.nlm.nih.gov/23028579/
WEBPMCID: PMC3444447. DOI: 10.1371/journal.pone.0044679. Abstract. It is known that sperm samples from recurrent pregnancy loss (RPL) couples have an increase in their sperm DNA fragmentation (SDF), but no studies have been performed in order to identify differences between single stranded SDF (ssSDF) and double stranded SDF (dsSDF) in these …
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Double Stranded Sperm DNA Breaks, Measured by Comet …
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0044679
WEBSep 17, 2012 · Abstract. It is known that sperm samples from recurrent pregnancy loss (RPL) couples have an increase in their sperm DNA fragmentation (SDF), but no studies have been performed in order to identify differences between single stranded SDF (ssSDF) and double stranded SDF (dsSDF) in these patients. This could be relevant because the …
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(PDF) Double-stranded sperm DNA damage is a cause of
https://www.researchgate.net/publication/331402841_Double-stranded_sperm_DNA_damage_is_a_cause_of_delay_in_embryo_development_and_can_impair_implantation_rates
WEBFeb 1, 2019 · The results show that dsSDF could be the main type of DNA damage that affects embryo development in intracytoplasmic sperm injection cycles, probably due to motility-based sperm selection in this...
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