Keyword Analysis & Research: pyrosequencing
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Pyrosequencing - Wikipedia
https://en.wikipedia.org/wiki/Pyrosequencing
WebPyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released.
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Pyrosequencing - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/neuroscience/pyrosequencing
WebPyrosequencing is an alternative sequencing technique of small DNA fragments based on the sequencing-by-synthesis principle. Compared to the Sanger method, pyrosequencing is less costly and time consuming, although the applications of this technique do not completely overlap with those of the conventional method.
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What is Pyrosequencing? - News-Medical.net
https://www.news-medical.net/life-sciences/What-is-Pyrosequencing.aspx
WebOct 31, 2018 · Pyrosequencing is a method of DNA sequencing that detects light emitted from nucleotide bases. By comparing the relative amount of light emitted, the sequence is uncovered.
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Pyrosequencing Technology and Platform Overview - QIAGEN
https://www.qiagen.com/us/knowledge-and-support/knowledge-hub/technology-and-research/pyrosequencing-resource-center/pyrosequencing-technology-and-platform-overview
WebPyrosequencing — the unique detection and quantification technology Characterization of complex DNA modifications underlying gene expression patterns requires quantifiable sequence data. Pyrosequencing is a sequence-based detection technology that enables rapid and accurate quantification of sequence variation.
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DNA methylation analysis by pyrosequencing | Nature Protocols
https://www.nature.com/articles/nprot.2007.314
WebSep 6, 2007 · Pyrosequencing is a sequencing-by-synthesis method that quantitatively monitors the real-time incorporation of nucleotides through the enzymatic conversion of released pyrophosphate into a ...
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5.12: Pyrosequencing - Biology LibreTexts
https://bio.libretexts.org/Bookshelves/Introductory_and_General_Biology/Biology_(Kimball)/05%3A_DNA/5.12%3A_Pyrosequencing
WebMay 14, 2022 · Its method is called pyrosequencing or sequencing by synthesis. It works like this. The DNA to be sequenced is broken up into fragments of ~100 base pairs and denatured to form single-stranded DNA (ssDNA). Single ssDNA fragments are attached to microscopic beads, which are separated from each other.
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Pyrosequencing | SpringerLink
https://link.springer.com/protocol/10.1385/1-59259-752-1:211
WebPyrosequencing, a bioluminometric DNA sequencing technique based on sequencing by synthesis, is emerging as a widely applicable tool for detailed characterization of nucleic acids (1–3). This technique relies on the real-time detection of inorganic pyrophosphate (PPi) released on successful incorporation of nucleotides during DNA synthesis.
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Pyrosequencing: History, biochemistry and future - ScienceDirect
https://www.sciencedirect.com/science/article/pii/S0009898105004274
WebJan 1, 2006 · Pyrosequencing is a DNA sequencing technology based on real-time detection of DNA synthesis monitored by bioluminescence. Four enzymes are exploited by the technology and a fifth protein, SSB, can be included to enhance the quality of the obtained sequences and thereby prolong the read length.
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Fundamentals of Pyrosequencing | Archives of Pathology
https://meridian.allenpress.com/aplm/article/137/9/1296/193658/Fundamentals-of-Pyrosequencing
WebSep 1, 2013 · Pyrosequencing is less complex, involves fewer steps, and has a superior limit of detection compared with Sanger sequencing. The fundamental basis of pyrosequencing is that pyrophosphate is released when a deoxyribonucleotide triphosphate is added to the end of a nascent strand of DNA.
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Pyrosequencing: powerful and quantitative sequencing technology
https://pubmed.ncbi.nlm.nih.gov/24510299/
WebJul 15, 2023 · Pyrosequencing is a sequencing-by-synthesis method for DNA analysis that has emerged as a platform not only for de novo sequencing applications, but also for quantitative analysis of genomic methylation, single-nucleotide polymorphisms, and allele quantification. In this unit, we describe a complete workflow from sample to result that is ...
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