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Engineering a Replication-Competent, Propagation-Defective
Sep 10, 2013 · rMERS-CoV-ΔE was a replication-competent, propagation-deficient virus and was only efficiently disseminated in cells expressing the E protein in trans. In the presence of transiently expressed E protein, rMERS-CoV-ΔE yielded maximum progeny viral titers of around 10 3 TCID 50 /ml. This modest yield could be improved by the generation of cell ...
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Replication-Competent Influenza A Viruses Expressing
To circumvent this requirement, replication-competent IAV expressing an easily traceable reporter protein can be used. Here we discuss the development and applications of recombinant replication-competent IAV harboring diverse fluorescent or bioluminescent reporter genes in different locations of the viral genome.
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Identification and elimination of replication-competent
Abstract. Adeno-associated virus (AAV) vector preparations are often contaminated with variable amounts of replication-competent AAV (rcAAV), which may influence the behavior of these vectors both in cultured cells and in animals. A packaging plasmid/vector plasmid system containing no significant homology and lacking the wild-type AAV p5 promoter was constructed to eliminate the production of …
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Replication-Competent AAV Testing - Creative Biogene
Replication-Competent AAV Testing. Replication-incompetent vector particles derived from AAV (adeno-associated virus) have been shown to mediate transfer and expression of heterologous genes (transgenes) into a variety of cells in vivo and in vitro. It has tremendous potential in both research and therapeutic applications.
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Detection of Replication Competent Lentivirus Using a qPCR
Although lentiviral vectors are ideal for delivery and stable integration of genes of interest into the host cell genome, they potentially pose risks to human health, such as integration-mediated transformation and generation of a replication competent lentivirus (RCL) capable of infecting non-target cells.
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What is replication competent lentivirus (RCL)? - Imanis
Replication competent lentiviruses (RCL) are virus particles capable of infecting cells and replicating to produce additional infectious particles. The existence of RCL in stable cell lines generated by lentiviral vector transduction is a safety concern. As such lentiviral vectors have been engineered to significantly reduce the likelihood of RCL production.
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Detection of Replication Competent Retrovirus and Lentivirus
Detection of Replication Competent Retrovirus Retroviral vectors based on murine leukemia viruses (MuLV) have been used in clinical investigations for Key words: Replication competent lentivirus . Gammaretrovirus-based retroviral vectors (subsequently referred and remain an attrac). Insertional mutagenesis is a rare but and Lentivirus
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What does replication competent mean?
Replication Competent Retrovirus. A retroviral vector that contains all the genes that are required for virion synthesis and is able to propagate after the host is infected.
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What is replication competent lentivirus (RCL)?
Exposure to replication-competent lentivirus (RCL) is a theoretical safety concern for individuals treated with lentiviral gene therapy. For certain ex vivo gene therapy applications, including cancer immunotherapy trials, RCL detection assays are used to screen the vector product as well as the vector-transduced cells.
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